Figure 3. Role of LOX-5 metabolites in ACSL4 effects on the mTOR pathway.

MCF-7 Tet-Off/ACSL4 cells were incubated in the presence or absence of zileuton (LOX-5 inhibitor, 500 μM) for 24 h. Whole cell extracts were obtained as described in Materials and Methods. Western blot was performed using the indicated antibodies. Representative blots A. and integrated optical density of protein levels B. quantified and normalized with the corresponding β-tubulin signal. Data represent the means of percentages of inhibition ± SD of three independent experiments. ***p < 0.001 and **p < 0.01 vs. control cells.