Skip to main content
. 2015 Oct 19;6(40):42632–42650. doi: 10.18632/oncotarget.5822

Figure 5. ACSL4 effects on the mTOR pathway in T47D cells.

Figure 5

T47D cells were transfected with the plasmid containing ACSL4 cDNA (T47D-ACSL4) or empty plasmid (T47D empty vector). Forty-eight hours post transfection, cells were selected with G418 (500 μg/ml) antibiotic. One month post selection, whole cell extracts were obtained and analyzed by Western blot using the indicated antibodies. Representative blots A. and integrated optical density of protein levels B. quantified and normalized with the corresponding β-tubulin signal. Data represent the means of fold changes (T47D-ACSL4 vs. T47D empty vector) ± SD of three independent experiments. Dotted line indicates control values. a, b, c, d, e and f: ***p < 0.001 vs. T47D empty vector cells.