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. 2015 Oct 13;6(40):42667–42686. doi: 10.18632/oncotarget.5626

Figure 2. Akt1 regulates formation and maintenance of NSCLC LCS.

Figure 2

A. Number of primary LCSs generated from NCI-H460 cells or the corresponding cells infected with lentivirus expressing shRNAs to Akt1 (clones #1, #2, #3, respectively). Data are shown as mean ± SD. ***p < 0.001. B. Analysis of size distribution (μm) of LCSs generated from NCI-H460 or NCI-H460-shAkt1 cells by phase-contrast microscopy *p < 0.05. C. Number of primary LCSs generated from PEd/10 cells or the corresponding cells interfered for Akt1 (clones #1, #2, #3, respectively) *p < 0.05. D. Analysis of size distribution (μm) of LCSs generated from PEd/10 cells by phase-contrast microscopy ***p < 0.001. E. Representative analysis of primary LCSs generated from PEd/10 cells or the corresponding cells interfered for Akt1 during serial passages (n = 10). F. Left, relative mRNA expression of stemness genes by Q-RT-PCR in NCI-H460 cells and derivatives; right, relative mRNA expression of stemness genes by Q-RT-PCR in PEd/10-scr cells and derivatives.