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. 2015 Oct 23;6(40):42825–42837. doi: 10.18632/oncotarget.5739

Figure 1. CRC cells induce IL-8 production in MSCs.

Figure 1

A. The expression of pro-angiogenic genes by MSCs treated with recombinant IL-1β (30 ng/mL) and TNF-α (20 ng/mL) were determined using RNA-Seq data. The results are presented as a histogram. B. IL-8 protein levels in culture media determined by ELISA in MSCs pretreated with recombinant IL-1β (30 ng/mL) and TNF-α (20 ng/mL) for 36 h. The results are presented as the mean values from three independent experiments (**, p < 0.01). C. In a CRC cell/MSC transwell system, MSCs were co-cultured with CRC cells (SW480, LS174T and HT29). After 36 h, IL-8 mRNA expression was measured using qRT-PCR and normalized to β-actin mRNA (**, p < 0.01). D. IL-8 protein levels in culture media determined by ELISA in CRC cells and MSCs before and after co-culture for 36 h. The results are presented as the mean values from three independent experiments (**, p < 0.01). E. MSCs and SW480 were co-cultured in a transwell system and a direct contact system separately for 36 h, and IL-8 expression in SW480 and MSCs was measured using qRT-PCR. F. IL-8 protein levels in culture media determined by ELISA in a transwell system and a direct contact system of MSCs and SW480 for 36 h. The results are presented as the mean values from three independent experiments.