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. 2015 Oct 23;6(40):42825–42837. doi: 10.18632/oncotarget.5739

Figure 3. MSCs promote endothelial tube formation through IL-8 secretion.

Figure 3

A. HUVECs were cultured on Matrigel in the presence of conditioned medium from CRC cells, CRC cell/MSC co-cultures or CRC cell/shIL-8-MSC co-cultures for 4 h. The images show the tube-formation ability of HUVECs in different conditioned media. Scale bar, 100 μm. B. Capillary-like structures were evaluated by quantifying tube lengths and numbers using the Angiogenesis Analyzer module in the ImageJ toolkit. The results are presented as the mean values per field from at least five randomly selected fields from three independent experiments (*, p < 0.05).