| Kountourakis [10] |
2006 |
Colorectal |
106 |
17.0% |
Positive: Membranous staining: >1% of tumor cells stained. Cytoplasmic staining: 2+: moderate immunostaining in >10% of tumor cells and 3+: strong immunostaining in >10% of tumor cells. |
6 |
| Hayashi [11] |
2008 |
Gastric |
134 |
59.0% |
Positive: 2+ = moderate staining, and 3+ = strong staining. |
7 |
| Baiocchi [12] |
2009 |
Colorectal |
109 |
69.7% |
Cytoplasmic staining was scored as “0” with no staining or weak staining in <10% of tumor cells; membranous staining: “0” when there was no staining at all or membrane staining <10% of tumor cells; “1+” with perceptible membrane staining in >10% tumor cells; “2+” with weak-to-moderate staining of the entire membrane in more than 10% tumor cells; “3+” with strong staining of the entire membrane in >10% tumor cells. |
5 |
| Begnami [13] |
2011 |
Gastric |
191 |
34.0% |
Slices with scores of 8 or higher were classified as positive, and slices with scores lower than 8 were classified as negative. |
6 |
| Beji [4] |
2012 |
Colorectal |
110 |
74.5% |
HER3 expression was classified as weak, intermediate, or strong (analysis compared low vs. intermediate + strong). |
6 |
| Wu [9] |
2014 |
Gastric |
161 |
55.9% |
Scores 0 and 1 were considered negative, and scores 2 and 3 were considered positive for HER3. |
6 |
| Ledel [15] |
2014 |
Colorectal |
236 |
69.5% |
The intensity of staining was graded 0–3, where grade 0–1 was categorized as low, and grade 2–3 was categorized as high expression of membranous HER3. |
7 |
| Ema [14] |
2014 |
Gastric |
167 |
58.7% |
0 = no staining observed in invasive tumor cells, 1+ = weak, incomplete membrane staining in any proportion of invasive tumor cells, or weak, complete membrane staining in less than 10% of cells, 2+ = complete membrane staining that is non-uniform or weak but with obvious circumferential distribution in at least 10% of cells, or intense complete membrane staining in 30% or less of tumor cells, 3+ = uniform intense membrane staining of more than 30% of invasive tumor cells. |
6 |
| Tang [16] |
2015 |
Gastric |
114 |
66.7% |
0, if no staining was observed; 1+, if more than 10% of the tumor cells had weak staining on the membrane (or cytoplasm for HER3); 2+, if more than 10% of the tumor cells had moderate staining on the membrane (or cytoplasm for HER3); and 3+, if more than 10% of the tumor cells had strong staining on the membrane (or cytoplasm for HER3). |
5 |
| Seo [3] |
2015 |
Colorectal |
364 |
69.0% |
0 and 1+ were considered no-over-expression, and 2+ and 3+ were considered over-expression. |
7 |
| He [17] |
2015 |
Gastric |
271 |
20.7% |
Immunoreactive results of 2+ or 3+ were considered to be positive or high expression. By contrast, 0 or 1+ was evaluated as negative or low expression. |
7 |
