Skip to main content
. 2015 Nov 3;6(40):43033–43047. doi: 10.18632/oncotarget.5824

Figure 2. Overexpression of miR-150 inhibits hemin-induced erythroid differentiation of K562 cells.

Figure 2

K562 cells transiently transfected with miR-150 mimics or controls for the mimics were induced by hemin and evaluated at 0, 24, 36 or 48 h after induction. Real-time qRT-PCR analysis of miR-150 A. HBB B. HBE C. and HBG D. Note that U6 or GAPDH was used as the control gene and that results were normalized to the control group at 0 h. Representative images E. and statistics F. of benzidine staining of hemoglobin-containing cells. Statistics G. and representative images H. of flow cytometry analysis of CD235a-positive cells. All data represent mean ± SD (n = 3). *P < 0.05 and **P < 0.01 for each paired condition with and without miR-150 overexpression.