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. 2015 Nov 3;6(40):43033–43047. doi: 10.18632/oncotarget.5824

Figure 6. 4.1R is a direct target of miR-150 during terminal erythroid differentiation.

Figure 6

A. The highly conserved miR-150-binding motif in the 3′ UTR of 4.1R (EPB41) mRNA predicted from http://www.targetscan.org. The artificially mutated sequence of the 3′ UTR of 4.1R (mut-EPB41) was shown in capital letters. B. Relative luciferase activity of the indicated EPB41 reporter construct in 293T cells. Cells were transfected with empty luciferase reporter plasmid, or cotransfected with by EPB41 3′ UTR, mutated EPB41 3′ UTR (mut EPB41 3′ UTR) or MYB 3′ UTR recombinant luciferase reporter plasmid and miR-191 or miR-150 recombinant overexpression plasmid. The luciferase assay was performed at 48 h after transfection. Data was shown as mean ± SD; n = 3; **P < 0.01 compared with the negative control. C. Western blot for the change in expression of 4.1R and c-Myb at 48 h after hemin induction in stable miR-150-overexpressing and control K562 cell lines.