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. Author manuscript; available in PMC: 2016 Feb 25.
Published in final edited form as: J Mol Biol. 2012 Mar 13;418(5):331–349. doi: 10.1016/j.jmb.2012.02.019

Figure 6. In-line probing of mutated metF SAM-I riboswitch RNA segments.

Figure 6

In-line probing experiment on wild-type (a) and eP0 mutant (b). The area highlighted in the red rectangle box is assigned to the GU highlighted by the blue arrow in Figure 2. In the context of sequences truncated at AT1, is opposite the fraying terminus of the AT1 helix. For the wild type sequence, this region has already been partially protected in the absence of SAM and fully protected in the presence of SAM. For the P0 mutant, the cleavage in this area is still persistent in the presence of low concentration SAM, indicating residual ON conformer formation.