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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Arthritis Rheumatol. 2016 Mar;68(3):730–739. doi: 10.1002/art.39453

Figure 3.

Figure 3

ApoER2 and β2GPI-apoER2 interaction are required for the attenuation of trophoblast migration by aPL.

A. HTR-8/SVneo cells were treated with PBS (NT), NHIgG (100 μg/ml), aPL (100 μg/ml), or aPL+sBD1 (50 μg/ml) for 24 h during scratch assays. Migration was expressed relative to migration with NT (N=4, Mean±SEM, *p<0.05 vs. NT, †p<0.05 vs. aPL alone). B. Scratch assays were performed in HTR-8/SVneo cells transfected with control siRNA or apoER2 siRNA in the presence of various concentrations of NHIgG of aPL (0-200 μg/ml) for 24 h. Migration was expressed relative to migration of NHIgG-treated cells (N=10, Mean±SEM, *p<0.05 vs. NHIgG). C. Scratch assays were performed in HTR-8/SVneo cells transfected with control siRNA or apoER2 siRNA in the presence of control IgG (Con IgG) or anti-β2 GPI monoclonal antibodies (FC1 or 3F8) Migration was expressed relative to migration of Control IgG-treated cells (N=10, Mean±SEM, *p<0.05 vs. NHIgG).