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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Traffic. 2016 Jan 10;17(3):230–244. doi: 10.1111/tra.12354

Figure 8. Reabsorption of ntcp-containing ruffles occurs at sites of colocalization between ntcp and fluorescent EGF.

Figure 8

High magnification live imaging was performed as in Figure 7C after the addition of fluorescent EGF (red) to HuH7 cells expressing ntcp-sfGFP (green; supplementary movies 4, 5). Ruffles were induced and were subsequently reabsorbed into the cells as highlighted by yellow lines. These reabsorption sites also contained coalescing fluorescent EGF that had been endocytosed by the cells. A and B provide two examples of this phenomenon. Some cells did not show visible expression of ntcp-sfGFP. Time in minutes is indicated.