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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Nat Genet. 2016 Jan 18;48(3):283–291. doi: 10.1038/ng.3486

Fig. 5. Piwi inhibits PRC2 binding to chromatin and PRC2-mediated H3K27 tri-methylation.

Fig. 5

(a) Circos plot shows H3K27m3 and E(z) binding patterns (enrichment scores calculated by ChIP-Seq counts within non-overlapping 10 Kb bins) in wild type and piwi mutant ovarian cells. Selected genes/peaks are indicated.

(b) Venn diagrams show overlaps of enriched regions between indicated ChIP-Seq datasets.

(c) Replicates of E(z) and H3K27m3 ChIP-Seq data in wild type and piwi mutant were compared.

(d) Confocal images of H3K27m3 and DAPI of wild type and piwi mutant ovarioles. H3K27m3 signals (normalized to DAPI signals) in piwi mutant were ~2-fold of wild type.

(e) Two-fold serial dilutions of histone extract from wild type and piwi mutant ovaries analyzed by immunoblotting to H3K27m3 and H3. H3K27m3 signals (normalized to H3 signals) in piwi mutant were ~2-fold of wild type.

(f) Gene ontology analysis of gene targets bound by H3K27m3. The bar graphs representing −lg of p-values.