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. Author manuscript; available in PMC: 2016 May 30.
Published in final edited form as: Nat Mater. 2015 Nov 30;15(3):326–334. doi: 10.1038/nmat4489

Figure 5. Nuclear localization of YAP is enhanced by faster stress relaxation, but decoupled from MSC fate.

Figure 5

a, Representative immunofluorescence staining for actin (green), nucleus (blue), and YAP (red) in MSCs cultured in the indicated conditions for a week. Scale bar is 10 μm. b, Quantification of the ratio of the concentration of nuclear YAP, to the concentration of YAP in the cytoskeleton. Nuclear YAP increases significantly with faster stress relaxation for both initial elastic moduli (Spearman’s rank correlation, p < 0.0001 for both). c, Quantification of percentage of D1 cells that stain positive for ORO as a function of the relative nuclear YAP. d, Quantification of ALP in differentiated D1 cells as a function of the relative nuclear YAP. No trend is observed in c and d. All data are shown as mean +/− s.d.