Figure 6. Protection of 1A6-treated baboons from collagen-initiated distal single platelet consumption.

4-mm internal diameter expanded-polytetrafluoroethylene (ePTFE) vascular graft coated with collagen was deployed into a chronic high flow arteriovenous (AV) shunt in healthy baboons. In parallel, control experiments were performed with tubing alone. Samples were drawn from the intraluminal coagulation marker concentration boundary layer by a syringe pump 1 cm downstream from acutely developing thrombi. 1 mM PPACK anticoagulant was infused at 1/5^th of the sample extraction rate 3 mm proximal to the sample port to prevent the sample port from occluding during the 1-hour study. Blood flow through the graft was maintained at a fixed rate of 100 mL/min for the entirety of each study by proximal clamping (A). Quantification of the six separate animal experiments for each treatment (mean±SEM) are reported (B). Significant rise in percent single platelet consumption immediately distal to collagen-coated ePTFE was seen between 10 to 20 min of perfusion in control animals (*, p = 0.0283; n = 6). 1A6-treated baboons maintained same single platelet levels at 20 min as at 10 min of perfusion (p = 0.9530; n = 6) significantly different from control at 20 min of perfusion (**, p = 0.0180; n = 6).