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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Insect Biochem Mol Biol. 2016 Jan 7;70:160–169. doi: 10.1016/j.ibmb.2016.01.001

Fig. 1.

Fig. 1

Extracellular calcium (Ca+2) enhances ecdysteroid hormone (ECD) production by ovaries after treatment with OEH (330 nM) or ILP3 (400 nM). (A) Ovaries were placed into saline containing 0, 1.7 or 8.5 mM Ca+2 plus OEH, ILP3, or no hormone (unstimulated) followed by measurement of ECD content in the medium after a 6 h incubation. A one-way ANOVA indicated that treatments overall differed significantly from one another (F8, 230 = 30.95, P < 0.0001). Bars with different letters indicate treatments significantly differed as determined by a post-hoc Tukey-Kramer HSD test (p≤0.05). (B) Ovaries were placed into standard saline containing 1.7 mM Ca+2 with or without 2 mM EGTA followed by addition of OEH, ILP3 or no hormone. ECD in the medium was then determined and statistically analyzed as in (A) (F5, 114 = 40.06, P < 0.0001). Bars in each graph show the mean ± standard error (SE) for each treatment.