Fig. 2.

The extracellular Ca⁺² channel blocker, gadolinium chloride (GdCl₃) (A), ionophore ionomycin (B), and SERCA inhibitor thapsigargin (C) differentially affect ECD production by ovaries following treatment with OEH or ILP3. In (A), ovaries were placed into standard saline containing 1.7 mM Ca⁺² without or with GdCl₃ (100 nM) followed by addition of OEH (330 nM), ILP3 (400 nM) or no hormone. (F_{8, 146} = 21.57, P < 0.0001). In (B), ovaries were placed in standard saline without or with ionomycin (2 and 20 μM) followed by addition of OEH, ILP3 or no hormone (F_{8, 210} = 41.80, P < 0.0001). In (C), ovaries were placed in saline containing 0–8.5 mM Ca⁺² plus thapsigargin (10 μM) by addition of OEH, ILP3 or no hormone (F_{8, 102} = 93.97, P < 0.0001). Determination of ECD in the medium, data presentation, and statistical analyses for each experiment were performed as in Fig. 1.