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. Author manuscript; available in PMC: 2017 Feb 23.
Published in final edited form as: Cell Rep. 2016 Feb 11;14(7):1787–1799. doi: 10.1016/j.celrep.2016.01.043

Figure 4. mRNA enrichment methods can bias mRNA abundance measurements.

Figure 4

(A) mRNA abundances measured by RNA-seq of Ribo-Zero-treated RNA compared to those measured by RNA-seq of total unselected RNA. Pearson R2 is indicated.

(B) Metagene analysis of RNA-seq read density in total unselected or mRNA-enriched RNA samples. Coding sequences were aligned by their stop codons, and RNA-seq reads were individually normalized by the mean reads within the ORF and then averaged with equal weight for each codon position across all ORFs (h’’j Eqn S15).

(C) mRNA abundances for mRNA-enriched samples relative to total unselected RNA, as a function of ORF length.

See also Figures S5–7 and Tables S3–4.