Table 1.

Between custom Multiplex assays repeatability of the selected tear cytokine concentrations.

Tear cytokine concentration	IL-1β	IL-1Ra	IL-6	IL-10	IL-12(p70)	TNF-α
Kit 1 by operator 1 (pg/ml)	33.6±20.1	25608.4±10854.5	156.4±91.9	61.5±39.7	239.0±131.3	416.9±301.0
Kit 2 by operator 2 (pg/ml)	25.2±15.9	7034.5±5118.9	120.8±77.4	41.1±27.2	115.6±59.1	282.0±164.7
p value	0.11	<0.01	0.14	0.09	0.15	0.80
Bias (pg/ml)	−8.41	−18573.9	−35.7	−20.3	−133.7	−134.9
CoR (pg/ml)	±43.2	±22666.6	±233.8	±92.3	±264.6	±640.1
LoA (pg/ml)	34.8; −51.7	4092.7; −41240.6	198.2; −269.5	71.9; −112.7	130.9; −398.3	505.2; −775.1

Data presented as mean± standard deviation (n=16).

CoR: coefficient of repeatability; LoA: Limits of agreement