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. Author manuscript; available in PMC: 2017 Feb 25.
Published in final edited form as: Adv Healthc Mater. 2015 Aug 25;4(14):2146–2153. doi: 10.1002/adhm.201500492

Figure 2.

Figure 2

Cellular viability, metabolic activity, and morphology in alginate:GelMA fibers. a,b) Micrographs showing the live (green) and dead (red) NIH-3T3 fibroblasts encapsulated within alginate:GelMA fibers with different compositions over 5 days of culture. c) Staining for α-actin showing the spreading of NIH-3T3 cells encapsulated within alginate:GelMA fibers after 5 days of culture. d) Cellular metabolic activity using MTS assay for 3.5 cm long fibers with the initial cell density of 106/ml. The results are normalized with respect to the values for day 1. Error bars represent standard deviation. e) Viability assay showing the high cellular viability after EDTA treatment for sacrificing the alginate network within the hydrogel fibers after 5 days of culture leaving behind a cell-laden GelMA fiber.