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Stringent heat map showing the percentage coverage of ICC disease genes commonly used to inform clinical practice (n = 43) at 20× read depth using M3 (MiSeq, 150 bp PE), M4 (NextSeq 500, 150 bp PE), deep whole exome sequencing (WES; HiSeq 2500, 125 bp PE), WES (HiSeq, 125 bp PE) and whole genome sequencing (WGS: HiSeq X, 150 bp PE) (gene coverage at 20×: dark red ≤98 %; dark green = 100 %)
