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. 2015 Oct 6;27(3):1063–1071. doi: 10.1007/s00198-015-3312-x

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Representative example of tartrate-resistant acid phosphatase–positive (TRAcP⁺) multinucleated cell (MNC) (three nuclei or more) (arrows) formed from CD14⁺ monocytes isolated from human buffy coat at day 21. Scale bar = 50 μm (a). Formation of TRAcP⁺ MNCs at culture days 14, 21, and 28. Data are expressed as total number of TRAcP⁺ MNCs per square centimeter (mean ± SEM). **p < 0.01. CLO = clonidine (b)