Table 1.
qPCR primer sequences
| Primer | Sequence 5′ → 3′ | Amplicon length (bp) |
Ensembl Gene ID |
|---|---|---|---|
| PGBD | TgCAgTTTgAAATCATTgCTATgTC AACAggCTTTTCTCTCCAATCTTAgA |
84 | ENSG00000113721 |
| TRAP | CACAATCTgCAgTACCTgCAAgAT CCCATAgTggAAgCgCAgATA |
128 | ENSG00000102575 |
| CTSK | CCATATgTgggACAggAAgAgAgTT TgCATCAATggCCACAgAgA |
149 | ENSG00000143387 |
| CALCR | gCATACCAAggAgAAggTCCATAT ATACTCCAgCCggTgTgTCAT |
78 | ENSG00000004948 |
| DCSTAMP | ATTTTCTCAgTgAgCAAgCAgTTTC AgAATCATggATAATATCTTgAgTTCCTT |
101 | ENSG00000164935 |
| ADRA2A | gCgAgATCAACgACCAgAAgT TCTggTAgATgCgCACgTAgA |
101 | ENSG00000150594 |
| ADRA2B | CCCATggCCTCTTCCAgTT gAAggCACggCggAAgT |
100 | ENSG00000222040 |
| ADRA2C | gTgTggTgCggCgTgTAC CCTgCgTCACCgACCAgTA |
102 | ENSG00000184160 |
For each gene, the first oligonucleotide sequence represents the forward primer and the second sequence, the reverse primer