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. 2016 Feb 25;11(2):e0149947. doi: 10.1371/journal.pone.0149947

Fig 8. Immunogold labeling of HIF-1α and VEGF protein in the yak heart.

Fig 8

A: Electron microscope images showing HIF-1α immunopositive silver grains in endothelial cells (arrowheads). B: HIF-1α immunopositive silver grains in cardiac muscle cells. C: No HIF-1α immunopositive silver grains in smooth muscle cells. D: Electron microscope images showing VEGF immunopositive silver grains in smooth muscle cells. E: VEGF immunopositive silver grains in endothelial cells (arrowheads). F: High magnification of VEGF immunopositive silver grains in endothelial cells (arrowheads). G: Some fine filaments were found in smooth muscle cells (boxed area). H: Smooth muscle cell has a higher electron density than endothelial cells. I, J: No gold particles were found in control sections. SMC: smooth muscle cell, EC: endothelial cell, EF: elastic fiber, SEF: secondary elastic fiber.