Table 1.

DGE sequencing quality assessment and statistics of alignment analysis.

Terms	Ct	T1	T2
Total Reads	20,732,818	23,720,082	21,372,324
Total Reads (%)	100.00%	100.00%	100.00%
Only adaptor reads	132,332	148,286	133,181
Only adaptor reads (%)	1.26%	1.23%	1.23%
Reads containing Ns	0	0	0
Reads containing Ns (%)	0.00%	0.00%	0.00%
Low-quality reads	548	642	623
Low-quality reads (%)	0.01%	0.01%	0.01%
Total clean reads	10,366,409	11,860,041	10,686,162
Total clean reads (%)	98.73%	98.76%	98.76%
Total Base Pairs	2,073,281,800	2,372,008,200	2,137,232,400
Total Base Pairs (%)	100.00%	100.00%	100.00%
Total Mapped Reads	16,889,894	19,132,922	17,367,263
Total Mapped Reads (%)	81.46%	80.66%	81.26%
Perfect match	13,559,314	15,274,836	13,877,373
Perfect match (%)	65.40%	64.40%	64.93%
≤ 2 bp mismatch	3,330,580	3,858,086	3,489,890
≤ 2 bp mismatch (%)	16.06%	16.27%	16.33%
Unique match	10,223,609	11,745,964	10,541,902
Unique match (%)	49.31%	49.52%	49.33%
Multi-position match	6,666,285	7,386,958	6,825,361
Multi-position match (%)	32.15%	31.14%	31.94%
Total Unmapped Reads	3,842,924	4,587,160	4,005,061
Total Unmapped Reads (%)	18.54%	19.34%	18.74%