TABLE 1.
Identification of 151 fastidious GNR by MALDI-TOF MS by applying direct colony transfer and standard Bruker interpretation criteria (species cutoff of 2.0; genus cutoff of 1.7)
| Organisma | No. (%) of isolates | No. (%) of MALDI-TOF MS resultsb: |
No. of reference spectrac | |||
|---|---|---|---|---|---|---|
| Species identification | Genus identification | No identification | Misidentification | |||
| Actinobacillus hominis | 2 | 2 | 0 | |||
| Aggregatibacter actinomycetemcomitans | 2 | 1 | 1 | 5 | ||
| Aggregatibacter aphrophilus | 10 | 5 | 3 | 2 | 3 | |
| Aggregatibacter sp. | 3 | 3 | 9 | |||
| Bordetella bronchiseptica | 1 | 1 | 9 | |||
| Bordetella petrii | 2 | 2 | 4 | |||
| Bordetella trematum | 1 | 1 | 3 | |||
| Capnocytophaga canimorsus | 3 | 1 | 2 | 1 | ||
| Capnocytophaga gingivalis | 1 | 1 | 1 | |||
| Capnocytophaga sputigena | 6 | 6 | 2 | |||
| Capnocytophaga sp. | 1 | 1 | 10 | |||
| Cardiobacterium hominis | 7 | 3 | 4 | 1 | ||
| Dysgonomonas capnocytophagoides | 1 | 1 | 0 | |||
| Dysgonomonas mossii | 1 | 1 | 0 | |||
| Eikenella corrodens | 13 | 10 | 3 | 2 | ||
| Haemophilus influenzae | 5 | 4 | 1 | 10 | ||
| Haemophilus parainfluenzae | 5 | 3 | 2 | 5 | ||
| Haemophilus sp. | 1 | 1 | 23 | |||
| Kingella denitrificans | 2 | 1 | 1 | 2 | ||
| Kingella kingae | 1 | 1 | 4 | |||
| Leptotrichia trevisanii | 1 | 1 | 0 | |||
| Moraxella atlantae | 1 | 1 | 1 | |||
| Moraxella canis | 1 | 1 | 2 | |||
| Moraxella lacunata | 2 | 1 | 1 | 1 | ||
| Moraxella nonliquefaciens | 5 | 5 | 3 | |||
| Moraxella osloensis | 10 | 1 | 5 | 4 | 7 | |
| Neisseria animaloris | 1 | 1 | 0 | |||
| Neisseria bacilliformis | 4 | 4 | 1 | |||
| Neisseria cinerea | 2 | 2 | 1 | |||
| Neisseria elongata | 9 | 1 | 7 | 1 | 2 | |
| Neisseria oralis | 1 | 1 | 0 | |||
| Neisseria shayeganii | 2 | 2 | 0 | |||
| Neisseria sicca | 1 | 1 | 3 | |||
| Neisseria subflava | 2 | 2 | 2 | |||
| Neisseria weaveri | 5 | 5 | 1 | |||
| Neisseria zoodegmatis | 5 | 5 | 1 | |||
| Neisseria sp. | 2 | 2 | 42 | |||
| Oligella urethralis | 6 | 3 | 3 | 2 | ||
| Pasteurella bettyae | 4 | 4 | 0 | |||
| Pasteurella canis | 1 | 1 | 3 | |||
| Pasteurella dagmatis | 1 | 1 | 1 | |||
| Pasteurella multocida | 11 | 11 | 10 | |||
| Pasteurella stomatis | 1 | 1 | 0 | |||
| Pasteurella sp. | 3 | 1 | 2 | 17 | ||
| Psychrobacter pulmonis | 1 | 1 | 0 | |||
| Psychrobacter sp. | 1 | 1 | 1 | |||
| Total | 151 (100) | 65 (43.0) | 49 (32.5) | 35 (23.2) | 2 (1.3) | |
a
Identification by 16S rRNA gene sequence analysis and biochemical methods according to de Melo Oliveira et al. (6).
b
MALDI-TOF MS identification applying direct transfer plus formic acid preparation, a genus cutoff value of 1.7, and a species cutoff value of 2.0 with Bruker database V.3.1.2.0 (3,995 entries).
c
Number of reference entries for the corresponding species and genus, respectively, included in Bruker database V.3.1.2.0 (3,995 entries).