TABLE 2.
Overall results for direct identification of pathogens from 171 polymicrobial BCs using the FilmArray BCID panel methoda
| Pathogen identified by the reference method (no. of isolates)b | No. (%) of isolates identified with FilmArray BCID panelc |
|---|---|
| Gram-negative bacteria (164) | 155 (94.5) |
| A. baumannii (12) | 12 (100) |
| Campylobacter rectus (1) | 0d |
| C. koseri (1) | 1 |
| C. freundii (2) | 1 |
| E. aerogenes (1) | 1 |
| E. cloacae (6) | 6 (100) |
| E. coli (38) | 38 (100) |
| K. oxytoca (2) | 2 |
| K. pneumoniae (36) | 36 (100) |
| M. morganii (3) | 0d |
| P. mirabilis (20) | 20 (100) |
| P. stuartii (1) | 0d |
| P. aeruginosa (33) | 33 (100) |
| S. marcescens (5) | 5 (100) |
| S. maltophilia (3) | 0d |
| Gram-positive bacteria (168) | 158 (94.0) |
| Bacillus cereus (2) | 0d |
| C. perfringens (2) | 0d |
| Clostridium tertium (1) | 0d |
| E. faecalis (43) | 42 (97.7) |
| E. faecium (11) | 11 (100) |
| E. gallinarum (3) | 3 |
| L. monocytogenes (1) | 1 |
| Parvimonas micra (1) | 0d |
| S. aureus (17) | 17 (100) |
| S. capitis (3) | 1 |
| S. epidermidis (47) | 47 (100) |
| S. haemolyticus (18) | 18 (100) |
| S. hominis (10) | 9 (90.0) |
| S. agalactiae (1) | 1 |
| S. anginosus (1) | 1 |
| S. gallolyticus (1) | 1 |
| S. mitis (3) | 3 |
| S. pneumoniae (2) | 2 |
| S. sanguinis (1) | 1 |
| Yeasts (30) | 30 (100) |
| C. albicans (19) | 19 (100) |
| C. glabrata (2) | 2 |
| C. krusei (1) | 1 |
| C. parapsilosis (6) | 6 (100) |
| C. tropicalis (2) | 2 |
| Total (362) | 343 (94.7) |
BC, blood culture; BCID, blood culture identification.
Isolates were identified using a reference method, which consisted of Gram stain microscopic examination and subcultures on routine medium, followed by MALDI-TOF MS analysis of bacterial or yeast colonies. Additionally, conventional phenotypical tests and/or sequencing analyses of the 16S rRNA and rpoB genes (for bacterial isolates) and the ITS1-5.8S-ITS2 rRNA gene regions (for yeast isolates) were performed whenever necessary.
FilmArray BCID panel testing of the polymicrobial BCs was performed according to the diagnostic algorithm presented in the study. According to the manufacturer's product insert, isolates identified as Enteric potentially belonged to enteric species, such as Cedecea davisae, Citrobacter spp., Cronobacter sakazakii, Enterobacter spp., Kluyvera ascorbata, Leclercia adecarboxylata, Raoultella spp., Salmonella spp., Shigella spp., and Yokenella regensburgei; isolates identified as Enterococcus potentially belonged to Enterococcus species, such as E. casseliflavus, E. durans, E. faecalis, E. faecium, and E. gallinarum; isolates identified as coagulase-negative staphylococci potentially belonged to Staphylococcus species, such as S. capitis, S. caprae, S. cohnii, S. epidermidis, S. haemolyticus, S. hominis, S. kloosii, S. lugdunensis, S. microti, S. nepalensis, S. saccharolyticus, S. saprophyticus, S. simiae, S. warneri, and S. xylosus; isolates identified as Streptococcus potentially belonged to Streptococcus species, such as S. anginosus, S. equinus, S. gallolyticus, S. gordonii, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. Percentages for the species with <5 isolates were not calculated.
Not identifiable by the method.