PCR results obtained for each patient with the four assays (negative or positive with CT value) according to the probability of PCP. Results were also presented depending on the type of pulmonary samples. *, Patients without P. jirovecii treatment at the time of pulmonary sampling. Each patient is represented by a dash. Blue dash, in house PCR; red dash, Bio-Evolution PCR; green dash, AmpliSens PCR; grey dash, MycAssay PCR.