TABLE 2.

PCR results of the 147 samples^a and concordance rate between the four PCR methods

PCR method and result	Amplisens PCR		MycAssay PCR		Bio-Evolution PCR
	Positive	Negative	Positive	Negative	Positive	Negative
In-house PCR (11)
No. positive	77	1	73	5	55	23
No. negative	5	64	0	69	0	69
Kappa concordance coefficient (95% CI)	0.92 (0.85–0.98)		0.93 (0.87–0.99)		0.69 (0.58–0.80)
Concordance, %	95.9		96.6		84.4
Bio-Evolution PCR
No. positive	55	0	55	0
No. negative	27	65	18	74
Kappa concordance coefficient (95% CI)	0.64 (0.53–0.76)		0.75 (0.65–0.86)
Concordance, %	81.6		87.8
MycAssay PCR
No. positive	72	1
No. negative	10	64
Kappa concordance coefficient (95% CI)	0.85 (0.77–0.93)
Concordance, %	92.5

^aThere is one missing result for the MycAssay PCR.