TABLE 5.
Summary of outcomes of discordant organism result investigations
Result and analyte | No. of results | No. of investigationsa |
|||
---|---|---|---|---|---|
Comparator result confirmed | FilmArray result confirmed | Both results incorrectb | Inconclusive | ||
BCID FP results | |||||
Enterococcus | 4 | 1 | 3 | ||
Staphylococcus | 12 | 11 | 1 | ||
S. aureus | 4 | 0 | 1 | 3 | |
Streptococcus | 5 | 1 | 1 | 3 | |
S. pneumoniae | 2 | 1 | 1 | ||
S. pyogenes | 1 | 1 | |||
A. baumannii | 5 | 5 | |||
Enterobacteriaceae | 4 | 1 | 3 | ||
E. cloacae complex | 3 | 1 | 2 | ||
E. coli | 5 | 1 | 2 | 2 | |
K. pneumoniae | 9 | 4 | 3 | 2 | |
K. oxytoca | 1 | 1 | |||
S. marcescens | 1 | 1 | |||
P. aeruginosa | 1 | 1 | |||
C. albicans | 4 | 4 | |||
C. glabrata | 2 | 2 | |||
C. parapsilosis | 2 | 2 | |||
Total | 65 | 12 | 18 | 5 | 30 |
% of total FP results | 18 | 28 | 8 | 46 | |
BCID FN results | |||||
Enterococcus | 3 | 3 | |||
Staphylococcus | 28 | 20 | 1 | 7 | |
S. aureus | 4 | 1 | 2 | 1 | |
Streptococcus | 5 | 3 | 2 | ||
S. pneumoniae | 1 | 1 | |||
Enterobacteriaceae | 8 | 4 | 3 | 1 | |
E. cloacae complex | 1 | 1 | |||
E. coli | 3 | 3 | |||
K. pneumoniae | 3 | 1 | 1 | 1 | |
K. oxytoca | 5 | 1 | 4 | ||
S. marcescens | 1 | 1 | |||
P. aeruginosa | 1 | 1 | |||
C. parapsilosis | 2 | 2 | |||
Total | 65 | 34 | 18 | 1 | 12 |
% of total FN results | 52 | 28 | 2 | 18 |
The method used was investigation of possible sample mix-ups, reculturing from frozen aliquots of the blood culture bottle fluid, sequencing directly from the bottle if isolated organisms were expected, or sequencing from resubcultured isolated organisms if more than one isolate per bottle was present. For bacterial resolution, 16S rRNA gene sequences were analyzed and for yeast, ITS1 was analyzed. See Materials and Methods for further information. Three apparent blood culture bottle mix-ups and one apparent bottle selection error were resolved in favor of BCID.
See Results for further information regarding the indicated organism.