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. 2016 Feb 25;54(3):687–698. doi: 10.1128/JCM.01679-15

TABLE 5.

Summary of outcomes of discordant organism result investigations

Result and analyte No. of results No. of investigationsa
Comparator result confirmed FilmArray result confirmed Both results incorrectb Inconclusive
BCID FP results
    Enterococcus 4 1 3
    Staphylococcus 12 11 1
    S. aureus 4 0 1 3
    Streptococcus 5 1 1 3
    S. pneumoniae 2 1 1
    S. pyogenes 1 1
    A. baumannii 5 5
    Enterobacteriaceae 4 1 3
    E. cloacae complex 3 1 2
    E. coli 5 1 2 2
    K. pneumoniae 9 4 3 2
    K. oxytoca 1 1
    S. marcescens 1 1
    P. aeruginosa 1 1
    C. albicans 4 4
    C. glabrata 2 2
    C. parapsilosis 2 2
    Total 65 12 18 5 30
    % of total FP results 18 28 8 46
BCID FN results
    Enterococcus 3 3
    Staphylococcus 28 20 1 7
    S. aureus 4 1 2 1
    Streptococcus 5 3 2
    S. pneumoniae 1 1
    Enterobacteriaceae 8 4 3 1
    E. cloacae complex 1 1
    E. coli 3 3
    K. pneumoniae 3 1 1 1
    K. oxytoca 5 1 4
    S. marcescens 1 1
    P. aeruginosa 1 1
    C. parapsilosis 2 2
    Total 65 34 18 1 12
    % of total FN results 52 28 2 18
a

The method used was investigation of possible sample mix-ups, reculturing from frozen aliquots of the blood culture bottle fluid, sequencing directly from the bottle if isolated organisms were expected, or sequencing from resubcultured isolated organisms if more than one isolate per bottle was present. For bacterial resolution, 16S rRNA gene sequences were analyzed and for yeast, ITS1 was analyzed. See Materials and Methods for further information. Three apparent blood culture bottle mix-ups and one apparent bottle selection error were resolved in favor of BCID.

b

See Results for further information regarding the indicated organism.