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CSF flow cytometry gating CSF cells (forward scatter [FSC] and side scatter [SCC]), showing a poor definition of cell subsets due to cryptococci (a1), CD45 and LIVE/DEAD stain are used to separate cells (a2), FSC-SSC plot of cryptococci (b), FSC-SSC plot of CSF leukocytes (c), and FSC-SSC plot of dead CD45+ cells.
