TABLE 2.
Effect of nonantibiotic agents on a preformed biofilm produced by bacteria isolated from mares with clinical disease
| Species from equine uterus (no. of isolates) | Nonantibiotic agentb | % reduction in biofilm biomass from untreated control (mean ± SEM) | Log decrease in CFU from untreated control (mean ± SEM) |
|---|---|---|---|
| E. coli (10) | NAC | 71.4 ± 5.2b | No CFU detected to >7 log decreaseb |
| H2O2 | 74.8 ± 5.9b | No CFU detected to >7 log decreaseb | |
| Tris-EDTA | 57.9 ± 7.2 | 2.6 ± 0.23b | |
| THAM-EDTA | 29.9 ± 8.1 | 1.1 ± 0.09 | |
| K. pneumoniae (10) | NAC | 28.7 ± 10.7 | 3.3 ± 0.6b |
| H2O2 | 44.3 ± 7.1 | No CFU detected to >7 log decreaseb | |
| Tris-EDTA | 17.2 ± 7.9 | 0.47 ± 0.3 | |
| THAM-EDTA | 18.4 ± 5.9 | 0.35 ± 0.7 | |
| P. aeruginosa (10) | NAC | 12.6 ± 32.1 | No CFU detected to >8 log decreaseb |
| H2O2 | 50.1 ± 14.6 | 1.6 ± 0.4 | |
| Tris-EDTA | 12.4 ± 30.9 | 1.5 ± 0.14 | |
| THAM-EDTA | −14.2 ± 32.9 | 0.8 ± 0.09 |
a
Agents were 3.3% N-acetylcysteine (NAC), 1% hydrogen peroxide, 50 mM Tris plus 3.5 mM EDTA (Tris-EDTA), and 20 mM THAM-8 mM EDTA (THAM-EDTA).
b
Significant difference (P < 0.05) compared to the untreated control.