FIG 3.

RT-QuIC endpoint dilution analyses of brain tissues from H-BSE-affected cattle using bank vole rPrP^Sen 23–230, M₁₀₉. The designated dilution of brain tissue from H-BSE cattle number 1, 2, or 3 and uninfected cattle (n = 2) were used to seed RT-QuIC reaction mixtures in the presence of 0.001% SDS and 300 mM NaCl using BV rPrP^Sen 23–230, M₁₀₉ as the substrate. Each trace indicates the average fluorescence (y axes) from four replicate wells seeded with the same brain homogenate dilution. Similar results were observed in two independent experiments. The calculated 50% seeding dose (SD₅₀) per mg of brain tissue is indicated for each brain sample tested.