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. 2016 Feb 25;54(3):676–686. doi: 10.1128/JCM.02731-15

FIG 4.

FIG 4

RT-QuIC detection of C-, L-, and H-BSE prion seeding activity in brain samples using multiple rPrPSen substrates. Quadruplicate RT-QuIC reaction mixtures were seeded with 10−4 brain tissue dilutions from uninfected (gray lines), C-BSE-affected (green lines), L-BSE-affected (red lines), and H-BSE-affected (blue lines) cattle in the presence of 0.001% SDS. A final concentration of either 300 mM NaCl (BV rPrPSen 23–230, M109 [A], BV rPrPSen 90–230, M109 [B], BV rPrPSen 23–230, I109 [C], Hu-BV rPrPSen 23–230, M109 [D], Ha rPrPSen 23–231 [G], Ha rPrPSen 90–231 [H], Sh rPrPSen ARQ 25–234 [I], VRQ 25–234 [J], ARR 25–234 [K], and Ha-S rPrPSen 23-234 [L]) or 130 mM NaCl (Mo rPrPSen 23–231 [E] and Hu rPrPSen 23–231 [F]) was used. Traces from representative RT-QuIC experiments are shown as the average of ThT fluorescence (y axes) from four replicate wells.