FIG 6.
Detection and discrimination of C-, L-, and H-BSE prion seeding activity by RT-QuIC using BV rPrPSen 23–230, M109 and Sh rPrPSen ARR 25–234. Serial dilutions of brain homogenates (10−3, 10−4, and 10−5) from three C-BSE-, three L-BSE-, and three H-BSE-affected cattle and one uninfected animal were tested by RT-QuIC. The same brain homogenate dilutions (circles, triangles, and squares indicate individual animals) were used to seed reaction mixtures with either BV rPrPSen 23–230, M109 (dark shades of green, red, and blue) or Sh rPrPSen ARR 25–234 (light shades of green, red, and blue). Representative data from at least two independent experiments are shown. The traces are the average fluorescence values (y axes) results from of four replicate wells.