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. 2016 Feb 26;7:179. doi: 10.3389/fmicb.2016.00179

FIGURE 2.

FIGURE 2

(A) Phenotypic analysis of the ability for the pBBR empty vector, the native siaD gene, and the mutated A-site siaDG140A or I-site siaDR130A alleles to restore aggregation to the ΔsiaD mutant during growth with 3.5 mM SDS. (B) Quantitative analysis of the percentage of area covered by macroscopic aggregates of different strains. (C) Transcription of cupA1 assessed with a cupA::lacZ reporter for the PAO1 pBBR plasmid control, the PAO1 pBBR[CC3396] strain encoding a functional c-di-GMP specific phosphodiesterase from Caulobacter crescentus, the ΔsiaD mutant, and ΔsiaD pBBR[siaD] complemented strain during growth in 10 mM succinate (black bars) or 3.5 mM SDS (white bars). Bars represent the mean value from three individual experiments and are accompanied by the individual data points. Statistical significance is indicated (∗p < 0.001, ∗∗p < 0.001, ∗∗∗p = 0.03).