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. 2016 Feb 26;7:179. doi: 10.3389/fmicb.2016.00179

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Copyright © 2016 Colley, Dederer, Carnell, Kjelleberg, Rice and Klebensberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Structural prediction of the cupA1 mRNA leader sequence using mFold (Zuker, 2003) with predicted primary (P) and secondary (S) RsmA binding sites. (B) Phenotypic analysis of strains PAO1 and PAO1::rsmA and the impact of RsmA induction (+) on macroscopic cell aggregation during growth with SDS. (C) Quantitative analysis of the percentage covered by macroscopic aggregates of different strains. Bars represent the mean value from three individual experiments and are accompanied by the individual data points. Statistical significance is indicated (^∗p = 0.001).