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. 2016 Feb 26;7:179. doi: 10.3389/fmicb.2016.00179

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Copyright © 2016 Colley, Dederer, Carnell, Kjelleberg, Rice and Klebensberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The effect of RsmA overexpression on the stability of cupA mRNA as determined by qRT-PCR. Data were normalized by the use of equivalent mRNA and analyzed by the 2^-ΔCt method. (B) Relative abundance of cupA1 mRNA during growth in SDS for strains PAO1, PAO1::rsmA and the mutant strains ΔsiaA and ΔsiaD. Data were normalized to the proC housekeeping gene by the 2^-ΔΔCt method. Bars represent the mean value from three individual experiments and are accompanied by the individual data points. Statistical significance compared to PAO1 is indicated (^∗p ≤ 0.01).