FIGURE 5.

Protein-RNA binding studies. Surface plasmon resonance with purified RsmA-His6 protein (961 RU ± 18.5 RU) immobilized on an NTA sensor chip (GE Healthcare) and 200 nM of the in vitro generated RNAs as interaction partners, namely the cupA1 RNA, the cupA1[AAA] RNA harboring a GGA to AAA mutation of the RsmA binding site overlapping with the SD sequence, the positive control RNA rsmZ and the negative control RNA lolB. The response units (RU), which indicate direct binding to RsmA, were normalized to the molecular weight of the rsmZ RNA. The start of sample injection (black arrow) and the end of sample injection (gray arrow) are indicated. Detailed information about the experimental setup and data analysis can be found in the Supplementary Material.