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. 2015 Dec 2;4(1):70–79. doi: 10.1002/iid3.95

Figure 1.

Figure 1

Serglycin‐dependent regulation of exogenously administered cytokines. (A) Peritoneal cell‐derived mast cells (PCMCs) from WT and serglycin−/− mice were stimulated with calcium ionophore A23187 (CI) in the presence of IL‐3, IL‐4, IL‐6, IL‐9, IL‐10, IL‐13, IL‐17A, eotaxin, GM‐CSF, IFN‐γ, and TNF‐α, all at 20 ng/ml. Samples were taken at 4 h post‐activation. Medium alone with addition of the cytokines was used as control. Cytokine levels in the supernatants were detected with a mouse cytokine antibody array. Layout of the mouse cytokine antibody array. (B) Cytokine array dots were quantified by densitometry. The duplicate values for each cytokine were normalized to positive control dots, represented on each membrane. Data are presented as means ± SEM (n = 2).