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. 2015 Dec 2;4(1):70–79. doi: 10.1002/iid3.95

Figure 4.

Figure 4

Degradation of endogenous IL‐6 is abrogated in serglycin−/− mast cell cultures. (A and B) WT and serglycin−/− PCMCs were degranulated by IgE receptor crosslinking (IgE/Ag) or by calcium ionophore A23187 (CI). Non‐stimulated cells were used as controls. The levels of endogenously produced IL‐6, 1, 4, and 24 h after stimulation, were analyzed by ELISA. Data are from representative experiments and are expressed as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. (C) IL‐6 mRNA levels in control mast cells (left panel), mast cells stimulated by IgE receptor crosslinking (IgE/Ag; middle panel) and mast cells stimulated by calcium ionophore (CI; right panel). Mast cells from either WT or serglycin−/− (SG−/−) mice were analyzed. Results are expressed as mRNA expression relative to the housekeeping gene (Hprt) and relative to controls. Note that the levels of IL‐6 gene expression were not significantly different between WT and serglycin−/− mast cells.