Table 1.

Protease activities in mast cell releasates1

	IgE/Ag			CI
	WT	Serglycin−/−	P‐value	WT	Serglycin−/−	P‐value
Chymase‐like2	32.2 ± 1.54	0.18 ± 0.43	<0.001	204 ± 12.4	16.7 ± 1.38	<0.001
Tryptase‐like2	301 ± 6.67	8.83 ± 1.19	<0.001	2360 ± 48.8	31.5 ± 1.44	<0.001
CPA‐like3	−1.42 ± 0.07	−0.09 ± 0.08	<0.001	−5.25 ± 0.02	0.24 ± 0.01	<0.001

¹WT or serglycin^−/− PCMCs were activated with IgE‐receptor crosslinking (IgE/Ag) or calcium ionophore A23187 (CI). Supernatants were collected after 1 h and analyzed for chymase‐, tryptase‐, and CPA‐like activities by adding fluorogenic or chromogenic substrates. Experiments were performed in triplicates.

²Activities are given as mean ± SEM of raw fluorescence units (RFU)/(min × 10⁶ cells).

³Activities are given as mean ± SEM of ΔmOD/(min × 10⁶ cells). Note that hydrolysis of the CPA substrate results in a decreased absorbance.