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. 2016 Feb 26;6:22229. doi: 10.1038/srep22229

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(a) Temperature profile with no Ca²⁺ addition (black) or 5 mM Ca²⁺ addition (red); (b) pH profiles with no Ca²⁺ addition (black) or 5 mM Ca²⁺ addition (red); (c) stability of recombinant PFA at 90, 100 °C with no Ca²⁺ addition (black, blue) or 5 mM Ca²⁺ addition (red, green); (d) stability of recombinant PFA at pH 4.5 (black), 5.0 (red) or 5.5 (blue) in boiling water bath, with no Ca²⁺ addition.