Table 1. Mycobacterial proteins identified by LC-MALDI-MS/MS from the phagosomal fractions of BCG mono- and HIV-BCG co-infected cells.
| S# | BCG gene# | pI | MW (kDa) | Functional category | Product/Function (Known/probable/annotated) | Ortholog in M.tuberculosis H37Rv (Rv#) |
|---|---|---|---|---|---|---|
| Proteins Observed in fractions from BCG mono-infected cells | ||||||
| 1 | BCG1194c | 4.9 | 81.5 | intermediary metabolism and respiration | Involved de novo biosynthesis of Methionine | Rv1133c |
| 2 | BCG3477 | 10.7 | 11 | virulence, detoxification, adaptation | Unknown; Possible Anti-toxin VapB47 | Rv3407 |
| 3 | BCG3226c | 6.1 | 116.6 | information pathways | Probable ATP-dependent DNA helicase/Has both ATPase and helicase activities | Rv3201c |
| 4 | BCG0136c | 7.3 | 77.4 | cell wall and cell processes | Probable cation-transporter P-type ATPase B CtpB/Cation-transporting ATPase | Rv0103c (ctpB) |
| 5 | BCG0422c | 4.9 | 92.5 | virulence, detoxification, adaptation | Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1) | Rv0384c |
| 6 | BCG2367c | 6.1 | 46.8 | intermediary metabolism and respiration | Probable dGTP triphosphohydrolase | Rv2344c |
| 7 | BCG0677c | 6.3 | 118.7 | information pathways | Probable exonuclease V (beta chain) RecB/Involved in homologous recombination. | Rv0630c |
| 8 | BCG2238c | 7.5 | 109.1 | intermediary metabolism and respiration | Glutamate-ammonia-ligase adenylyltransferaseGlnE/Regulatory protein involved in the regulation of glutamine synthetase activity | Rv2221c |
| 9 | BCG2457c | 5.4 | 74.6 | intermediary metabolism and respiration | Glutamine-dependent NAD(+) synthetaseNadE (NAD(+) synthase/Involved in biosynthesis of NAD | RV2438c |
| 10 | BCG1867 | 5.3 | 99.4 | intermediary metabolism and respiration | Probable glycine dehydrogenase GcvB/The glycine cleavage system catalyses the degradation of glycine. | Rv1832 |
| 11 | BCG1419c | 5.9 | 33.9 | conserved hypotheticals | Conserved hypothetical protein/Unknown | Rv1357c |
| 12 | BCG2119 | 6.5 | 58.9 | insertion seqs and phages | Conserved hypothetical protein/Unknown | Rv2100 |
| 13 | BCG0079c | 7.5 | 30.8 | cell wall and cell processes | Possible membrane protein/Unknown | Rv0048c |
| 14 | Mb2002c | 4.5 | 24.8 | cell wall and cell processes | Immunogenic protein Mpt64/Unknown. | Rv1980c |
| 15 | BCG3662c | 10.69 | 12 | information pathways | Iron-regulated H-NS-like protein Lsr2/Has DNA-bridging activity. | Rv3597c |
| 16 | BCG3174 | 5.2 | 85.3 | intermediary metabolism and respiration | Probable NADH dehydrogenase I (chain G) NuoG/Involved in aerobic|anaerobic respiration | Rv3151 |
| 17 | BCG1088 | 6.3 | 74.6 | cell wall and cell processes | Probable potassium-transporting P-type ATPase B chain KdpB | Rv1030 (kdpB) |
| 18 | BCG1391c | 6.5 | 70.1 | information pathways | Probable ATP-dependent helicase DinG/Probable helicase involved in DNA repair and perhaps also replication. | Rv1329c |
| 19 | BCG1182 | 4.99 | 52.1 | intermediary metabolism and respiration | Probable glucose-6-phosphate 1-dehydrogenase Zwf1 (G6PD)/Involved in pentose phosphate pathway (first step) | Rv1121 |
| 20 | BCG3756c | 9.6 | 16.3 | virulence, detoxification, adaptation | Possible toxin VapC48/Unknown | Rv3697c |
| 21 | BCG0453c | 6.1 | 23.2 | intermediary metabolism and respiration | Thiamine-phosphate pyrophosphorylaseThiE/Involved in thiamine biosynthesis | Rv0414c |
| 22 | BCG3326; BCG3362 | 8.7 | 28.5 | information pathways | Probable endonuclease VIII Nei/Involved in damage reversal of DNA | Rv3297 |
| 23 | BCG1573 | 5 | 63.1 | lipid metabolism | Probable fatty-acid-AMP ligase FadD25/Function unknown, but involvement in lipid degradation | Rv1521 |
| 24 | BCG0932 | 10.9 | 15.5 | Regulatory proteins | Possible transcriptional regulatory protein (possibly MarR-family)/Thought to be involved in transcriptional mechanism. | Rv0880 |
| 25 | BCG1357 | 5.4 | 65.1 | information pathways | Probable transcription termination factor Rho homolog/Facilitates transcription termination by a mechanism that involves rho binding to the nascent RNA, activation of rho’S RNA-dependent ATPase activity, and release of the mRNA from the DNA template | Rv1297 |
| 26 | BCG0937 | 8.7 | 39.7 | conserved hypotheticals | Conserved hypothetical protein/Unknown | Rv0885 |
| 27 | BCG0788 | 5.2 | 19.1 | conserved hypotheticals | Conserved protein/Unknown | Rv0738 |
| 28 | BCG2654c | 10 | 46.2 | conserved hypotheticals | Conserved protein/Unknown | Rv2627c |
| 29 | BCG0489c* | 7.11 | 105.2 | cell wall and cell processes | Probable conserved transmembrane transport protein MmpL4/Unknown. Thought to be involved in fatty acid transport. | Rv0450c |
| 30 | BCG2361; BCG2362 | 6.5 | 89.9 | cell wall and cell processes | Probable conserved transmembrane transport protein MmpL9/Unknown. Thought to be involved in fatty acid transport. | Rv2339 |
| Proteins Observed in fractions from HIV-BCG co-infected cells | ||||||
| 1 | BCG3770 | 4.78 | 70 | intermediary metabolism and respiration | 2-isopropylmalate synthase LeuA/Involved in leucine biosynthesis (at the first step) | Rv3710 |
| 2 | BCG3025c | 6.5 | 66 | intermediary metabolism and respiration | Acetolactate synthase (large subunit) IlvB1/Involved in valine and isoleucine biosynthesis (at the first step) | Rv3003c |
| 3 | BCG0626 | 10.5 | 7.5 | virulence, detoxification, adaptation | Possible antitoxin VapB26/Unknown | Rv0581 |
| 4 | BCG0391 | 8.2 | 41.3 | virulence, detoxification, adaptation | Probable chaperone protein DnaJ1/Acts as a co-chaperone. | Rv0352 |
| 5 | BCG2216c | 7.97 | 40.4 | intermediary metabolism and respiration | Probable transmembrane cytochrome C oxidase (subunit II) CtaC/Involved in aerobic respiration. | Rv2200c |
| 6 | BCG3007c | 12.4 | 22.1 | Information pathways | DNA-binding protein HU homolog HupB (histone-like protein)/This protein belongs to the histone like family of prokaryotic DNA-binding proteins which are capable of wrapping DNA to stabilize it, and prevent its denaturation under extreme environmental conditions. | Rv2986c |
| 7 | BCG0006; BCG0036 | 5.2 | 92.2 | Information pathways | DNA gyrase (subunit A) GyrA | Rv0006c |
| 8 | BCG3932 | 7.7 | 51 | cell wall and cell processes | ESX conserved component EccB1/Unknown. | Rv3869 |
| 9 | BCG0332* | 10.5 | 35.9 | cell wall and cell processes | ESX conserved component EccE3. ESX-3 type VII secretion system protein/Unknown. | Rv0292 |
| 10 | BCG3331c | 6.7 | 62 | intermediary metabolism and respiration | Probable glycerol-3-phosphate dehydrogenase GlpD2/Involved in aerobic respiration and oxidation of glycerol. | Rv3302c |
| 11 | BCG3470 | 5.6 | 87 | intermediary metabolism and respiration | Conserved protein/Function unknown; probably enzyme involved in cellular metabolism. | Rv3401 |
| 12 | BCG2921c | 7.7 | 84.5 | intermediary metabolism and respiration | Possible formate dehydrogenase H FdhF/Decomposes formic acid to hydrogen and carbon dioxide under anaerobic conditions in the absence of exogenous electron acceptors | Rv2900c |
| 13 | BCG0535c | 7.3 | 35.5 | conserved hypotheticals | Conserved protein/Function Unknown. | Rv0493c |
| 14 | BCG2433c | 6.5 | 28.4 | conserved hypotheticals | Conserved protein/Function Unknown. | Rv2417c |
| 15 | BCG1633* | 6.7 | 53.7 | intermediary metabolism and respiration | Probable L-aspartate oxidase NadB/Quinolinate biosynthesis. | Rv1595 |
| 16 | BCG1872c | 4.8 | 80.4 | intermediary metabolism and respiration | Malate synthase G GlcB/Involved in glyoxylate bypass (second step), an alternative to the tricarboxylic acid cycle | Rv1837c |
| 17 | BCG1689 | 4.9 | 88 | information pathways | Probable phenylalanyl-tRNAsynthetase, beta chain PheT/Charging PHE-tRNA | Rv1650 |
| 18 | BCG0447 | 5.04 | 72.9 | intermediary metabolism and respiration | Probable phosphate acetyltransferasePta/Involved at the last step (of two) in the conversion of acetate to acetyl-CoA | Rv0408 |
| 19 | BCG2957* | 5.5 | 158 | lipid metabolism | Phenolpthiocerol synthesis type-I polyketide synthase PpsE/Involved in phenolpthiocerol and phthioceroldimycocerosate (dim) biosynthesis: | Rv2935 |
| 20 | BCG1855 | 5.7 | 57.2 | intermediary metabolism and respiration | Probable acetolactate synthase IlvG/Valine and isoleucine biosynthesis (first step). | Rv1820 |
| 21 | BCG3661c | 5.6 | 93.5 | intermediary metabolism and respiration | Probable ATP-dependent protease ATP-binding subunit ClpC1/Hydrolyses proteins in presence of ATP. | Rv3596c |
| 22 | BCG3299 | 7.4 | 76.5 | cell wall and cell processes | Probable metal cation-transporting P-type ATPase C CtpC/Metal cation-transporting ATPase | Rv3270 |
| 23 | BCG0957 | 6.3 | 26 | lipid metabolism | Possible enoyl-CoA hydratase EchA6/Could possibly oxidize fatty acids using specific components | Rv0905 |
| 24 | BCG0976c | 10.7 | 44.9 | cell wall and cell processes | Divalent cation-transport integral membrane protein MntH (BRAMP)/H(+)-stimulated, highly selective, divalent cation uptake system. | Rv0924c |
| 25 | BCG2596 | 6.5 | 28.1 | conserved hypotheticals | Conserved protein/Function Unknown. | Rv2573 |
| 26 | BCG3443 | 9.7 | 48.8 | lipid metabolism | Possible triacylglycerol synthase/May be involved in synthesis of triacylglycerol | Rv3371 |
| 27 | BCG0992 | 7.8 | 83.5 | Information pathways | ATP dependent DNA ligase LigD/Involved in DNA double-strand break repair, by nonhomologous end joining (NHEJ). | Rv0938 |
| 28 | BCG1479 | 8.4 | 24.2 | cell wall and cell processes | Probable lipoprotein LprH/Function Unknown | Rv1418 |
| 29 | BCG1655 | 5.3 | 50.5 | intermediary metabolism and respiration | Probable pyruvate kinase PykA/Produces phosphoenol pyruvate in glycolysis | Rv1617 |
| 30 | BCG2858c | 5.3 | 18.9 | information pathways | Probable ribosome-binding factor aRbfA/Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). | Rv2838c |
| 31 | BCG2870c | 8.5 | 66.9 | intermediary metabolism and respiration | Possible magnesium chelatase/Function unknown; possibly introduces a magnesium ion into specific substrate/compound. | Rv2850c |
| 32 | BCG1671 | 4.7 | 78 | information pathways | Probable excinuclease ABC (subunit B - helicase) UvrB/Involved in nucleotide excision repair. | Rv1633 |
| 33 | BCG1667 | 4.78 | 98.4 | information pathways | Probable DNA polymerase I PolA/Involved in post-incision events. | Rv1629 |
| 34 | BCG3633c | 5.96 | 33.58 | intermediary metabolism and respiration | 3,4-DHSA dioxygenase/Catalyzes the extradiol cleavage of 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) | Rv3568c |
| 35 | BCG1816 | 5.1 | 152.8 | cell wall and cell processes | ESX conserved component EccC5. ESX-5 type VII secretion system protein/Function Unknown | Rv1783 |
| 36 | BCG2636c* | 8.96 | 35.16 | lipid metabolism | Probable acyltransferase/Catalyzes the acylation of the 6-position of the mannose residue linked to position 2 of the myo-inositol in phosphatylinositol mono- and DI-mannosides. | Rv2611c |
| 37 | BCG3639 | 9.4 | 21.9 | regulatory proteins | Transcriptional regulatory protein KstR (probably TetR-family)/Involved in transcriptional mechanism. Predicted to control regulon involved in lipid metabolism. | Rv3574 |
| 38 | BCG2891c | 8.69 | 42.9 | cell wall and cell processes | Membrane bound metalloprotease/Controls membrane composition | Rv2869c |
| 39 | BCG0602 | 10.63 | 41.24 | lipid metabolism | MannosyltransferaseMgtA/Involved in lipomannan (LM) biosynthesis | Rv0557 |
| 40 | BCG0733 | 4.69 | 77.20 | Information pathways | Probable elongation factor G FusA1 (EF-G)/This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. | Rv0684 |
| Proteins Observed in fractions from both mono- and co-infected cells | ||||||
| 1 | BCG1389c | 5.3 | 78.6 | intermediary metabolism and respiration | Unknown; probably involved in polysaccharide degradation | Rv1327c |
| 2 | BCG1368 | 4.7 | 59.2 | intermediary metabolism and respiration | Probable ATP synthase alpha chain AtpA | Rv1308 |
| 3 | BCG1370 | 4.5 | 53.09 | intermediary metabolism and respiration | Probable ATP synthase beta chain AtpD | Rv1310 |
| 4 | BCG1627 | 4.4 | 37.5 | intermediary metabolism and respiration | Probable biotin synthetase BioB/ Involved in biotin synthesis. | Rv1589 |
| 5 | BCG0389 | 4.5 | 66.8 | virulence, detoxification, adaptation | Probable chaperone protein DnaK (heat shock protein 70)/Acts as chaperone | Rv0350 |
| 6 | BCG2562c | 6.5 | 41.7 | intermediary metabolism and respiration | Probable chorismate synthase AroF/Involved in the synthesis of chorismate | Rv2540c |
| 7 | BCG2944c | 4.9 | 130.6 | cell wall and cell processes | Probable chromosome partition protein Smc/Plays an important role in chromosome structure and partitioning | Rv2922c |
| 8 | BCG0717 | 6 | 146.7 | information pathways | DNA-directed RNA polymerase (beta’ chain) RpoC | Rv0668 |
| 9 | BCG0716 | 4.67 | 129.2 | information pathways | DNA-directed RNA polymerase (beta chain) RpoB | Rv0667 |
| 10 | BCG3940c | 10.6 | 57.6 | cell wall and cell processes | ESX conserved component EccE2. ESX-2 type VII secretion system protein/Function unknown | Rv3885 |
| 11 | BCG0946 | 7.2 | 42.7 | Regulatory proteins | Possible transcriptional regulatory protein (possibly LuxR-family)/Thought to be involved in transcriptional mechanism. | Rv0894 |
| 12 | BCG2301c; BCG2302c | 6.3 | 25.4 | conserved hypotheticals | Possible membrane protein/Unknown | Rv2286c |
| 13 | BCG2962c* | 4.8 | 224 | lipid metabolism | Probable multifunctional mycocerosic acid synthase membrane-associated Mas/Catalyzes the elongation of N-fatty acyl-CoA with methylamalonyl-CoA (not malonyl-CoA) as the elongating agent to form mycocerosyl lipids | Rv2940c |
| 14 | BCG1539 | 9.3 | 49.8 | virulence, detoxification, adaptation | Peptidoglycan hydrolase/Unknown | Rv1477 |
| 15 | BCG2953 | 5 | 198.8 | lipid metabolism | Phenolpthiocerol synthesis type-I polyketide synthase PpsA/Involved in phenolpthiocerol and phthioceroldimycocerosate (dim) biosynthesis | Rv2931 |
| 16 | BCG2112c | 7.4 | 99.57 | information pathways | ATP-dependent DNA helicase HelY/DNA helicase activity | Rv2092c |
| 17 | Mb2007c | 9.3 | 32.8 | Regulatory proteins | Probable transcriptional regulatory protein (probably LysR-family)/Involved in transcriptional mechanism. | Rv1985c |
| 18 | BCG1085c | 5.8 | 92.7 | Regulatory proteins | Probable sensor protein KdpD/Member of the two-component regulatory system KDPD/KDPE involved in the regulation of the KDP operon. | Rv1028 |
| 19 | BCG0123 | 10.6 | 27.8 | cell wall and cell processes | Possible membrane protein/Unknown | Rv0090 |
| 20 | BCG1550 | 6.1 | 41.2 | cell wall and cell processes | Possible exported conserved protein/Unknown | Rv1488 |
| 21 | BCG3215c | 9.2 | 107.4 | cell wall and cell processes | Probable conserved transmembrane protein/Unknown | Rv3193c |
| 22 | BCG2500c | 8.7 | 88.2 | lipid metabolism | Probable glycerol-3-phosphate acyltransferase PlsB2/Involved in phospholipid biosynthesis | Rv2482c |
Note: *Corresponding protein belongs to IdeR regulon24.