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. 2015 Aug;1848(8):1671–1677. doi: 10.1016/j.bbamem.2015.04.015

Fig. 5.

Fig. 5

SDS-PAGE of chemically cross-linked AH2 in POPC and POPC/POPG (molar ratio 2:1) vesicles, at a lipid to protein ratio of 100:1. Cross-linking was carried out using DSS, followed by TCA precipitation for removal of lipids. Samples were run on a 16% tricine SDS-PAGE gel, and visualised using InstantBlue stain. Uncross-linked samples show a single band at the expected size with a molecular weight between 2 and 3.5 kDa, corresponding to monomeric AH2 peptide.