Table 1. Hydrodynamic properties for Hib PRP-native, Hib PRP-ADH and Hib PRP-TT derivatives.
Sample | so20,w (S) | ks mL.g−1 | 10−3 × Mwa g.mol−1 | 10−3 × Mzb g.mol−1 | [η]c mL.g−1 | [η]d mL.g−1 | [η]e mL.g−1 | ks/[η] | f/fo |
---|---|---|---|---|---|---|---|---|---|
PRP-native | 5.9 ± 0.2 | 400 ± 40 | 1200 ± 50 | 1250 ± 60 | 447 ± 14 | 445 ± 8 | 445 ± 6 | 0.9 ± 1 | 9.8 |
PRP-ADH | 6.0 ± 0.2 | 260 ± 40 | 240 ± 10 | 325 ± 20 | 275 ± 4 | 273 ± 3 | 275 ± 3 | 0.9 ± 2 | 3.3 |
PRP-TT | 30.0 ± 0.5 | 190 ± 10 | 7300 ± 420 | 7700 ± 390 | 225 ± 2 | 224 ± 6 | 224 ± 4 | 0.9 ± 1 | 6.4 |
TTf monomer | 7.6 ± 0.1 | 150 ± 5 | 5.7 ± 0.1 | 5.7 ± 0.1 | 1.3 | ||||
TTf dimer | 11.6 ± 0.2 | 270 ± 15 |
In phosphate-chloride buffer (pH = 6.8, I = 0.10).
aSedimentation equilibrium SEDFIT-MSTAR analysis.
bSedimentation equilibrium MFIT analysis.
cHuggins extrapolation procedure.
dKraemer extrapolation procedure.
eSolomon-Ciuta procedure.
ffrom ref. 33.