Figure 7. Functional analyses of HliR, an acyl-CoA dehydrogenase.

(a) Substrate specificity of recombinant acyl-CoA dehydrogenase HliR. (b) In vitro dehydrogenation of acyl-SNAC compounds by HliR. HPLC analyses of the enzymatic reaction mixtures (black lines) with the substrates 2a−d. The chromatograms were recorded at 254 nm.