Table 3. Factors affecting the stability and flexibility of EstSL3 and other cold active mesophilic or thermophilic counterparts.

Parameters	EstA	Axe2	LipPc	Est97	EstSL3
PDB code	3DOH	3W7V	4NS4	4AO6	–
Topt (°C)	95	50–60	25	35	30
Gly percent (%)	6.84	8.68	6.31	13.13	8.53
Pro percent (%)	6.84	4.11	4.73	4.63	3.32
Arg/(Arg+ Lys)	15/39	15/35	8/32	15/27	18/29
Number of salt bridges (<3.2 Å)	13	13	9	11	6
Number of hydrogen bonds	1025	973	607	487	126
Hydrogen bond percentage (%)	32.99	52.81	28.14	27.24	7.57
References	32	31	22	27	This study