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. 2016 Jan 27;10(2):1954–1959. doi: 10.1021/acsnano.5b05863

Figure 2.

Figure 2

Functionality of SH bonds of Zr-clusters in copolymerized structures. (a) Sketch of the lithographically structured polymers and the incubation with 10 mM Alexa647-maleimide. (b) Confocal images of the woodpile structures. The central image represents the DETC fluorescence in the green channel of the microscope (excited at 488 nm). The right one shows the specific signal of Alexa647-maleimide taken with the red channel (excited at 647 nm). The photoresist used for structuring was composed of 1 wt % ZrSH2. (c) Confocal images of one layer of the three-dimensional woodpile structures. Three different photoresists were used: pure PETA with 0.25 wt % DETC as a negative probe and PETA/DETC doped with 1 wt % ZrSH2 and 1 wt % Zr4, respectively. The large confocal image shows the signal from the Alexa647-maleimide attached to the mercapto groups in the polymer. The upper small image shows unspecific binding of Alexa647-maleimid to a woodpile structure without any Zr cluster, the lower small image shows unspecific binding to acrylates containing 1 wt % Zr4 (LSM images were scaled identically). (d) Bar diagram showing the background corrected fluorescence signal in the green channel (DETC fluorescence) and the red channel (Alexa647).