(a) Confocal real space imaging and BFP imaging
setup. (b) Scheme
illustrating the BFP radiation pattern created by a point-dipole emitter. p⃗, dipole moment; Φ, orientation angle of
dipole axis in sample plane; θ, emission angle; θs, incident angle complementary to θ; ϕ, azimuthal
angle in back aperture; r, radial distance from optical
axis.19 (c) Calculated BFP patterns of
an in-plane point dipole on an air–glass interface oriented
with Φ = 0° recorded for parallel (Φ = 0°, α
= 0° and Φ = 90°, α = 90°) and crossed
polarization (Φ = 0°, α = 90° and Φ =
90°, α = 0°), with α the orientation of the
analyzer transmission (detection polarization). The emission detected
for cross-polarization is due to polarization mixing caused by the
air–glass interface.