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Bacteria induced phagocytic cup formation in wild-type but not in far1− cells. Vegetative wild-type (A) and far1− (B) expressing LimEΔcoil-GFP (green) grown in D3-T medium were harvested, washed with PB buffer and settled in a 4 well chamber for 10 min. A 20 fold excess of pHrodo-labelled K. aerogenes (red) was added to the D. discoideum cells and the engulfment process was recorded by confocal microscopy.
